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Amino Labs FAQ

General information

What is Bioengineering / Genetic Engineering?

Biological/Genetic Engineering is an interdisciplinary area focusing on the application of engineering principles to biological systems in order to solve problems in the realm of sustainable food, materials, energy, and health. 

Similar to miniature factories, bacteria follow DNA programming that has been inserted into their "bodies" through genetic engineering and create products in response. Each bacterium produces a small quantity of the product which, when cultivated in large vessels, create significant amounts of pigments, medicine, plastic compounds, etc, that can be extracted and used by industries and individuals. As bacteria multiply rapidly and easily when fed sugars and kept in a controlled environment, creating products through genetic/biological engineering is sustainable and safe.

Thanks to the hard work of scientists around the world, programming DNA is improving our quality of life and keeping diseases at bay. Already in the hands of hundreds of millions of people each day, the number of genetically engineered products will continue to rise in the future. And now, you too can program bacteria! Amino Labs Kits have everything you need to bioengineer for the first time or the hundredth! 

By getting hands-on experience with genetic engineering and biotechnology, you will become immersed in some of the most cutting-edge science of the 21st century. Find out more hands-on and in more details by completing our Virtual Bioengineer online simulations.

You can also read more about bioengineering, Genetic engineering and biohacking in this blog post

How will I learn?

Learning and prototyping with genetic engineering and cells is starting to become accessible to newcomers of all ages and backgrounds thanks to dedicated scientists and kits such as the one you are about to use! The easiest way to learn a new science, hobby or topic is by trying it, hands-on.Our Amino Labs Kits make it easy to add a DNA program into living cells by following the instructions in this booklet and by using videos on Amino Labs' Youtube channel

Everything you need to complete the science in included; each ingredient in the kit is pre-measured and labelled for a stress-free experience. Our all-in-one stations decrease, setup time, mess, guesswork and the need to collect and calibrate multiple machines. The instructions are easy-to-follow for non-scientists and scientists alike.

An important addition to our ecosystem is our Virtual Bioengineer™ simulations developed with the educators at the Biobuilder Educational Foundation. Free 20 minutes guided experiences virtually replicates the hands-on procedures you will soon complete with your system. An easy way to practice using the DNA Playgrounds and Kits beforehand. Each simulator includes additional information on the manipulations, as well as deeper look into DNA and biological engineering. We recommend it strongly! Find the simulators here

Finally, the newest addition to our ecosystems is the Zero to Genetic Engineering Hero Beginner's book and lesson guides

The world's first beginner's guide to genetic engineering is here! With a combination of real-world, STEM hands-on activities and fundamental concepts, the Zero to Genetic Engineering Hero book has many levels of depth so you can choose how you want to learn. Are you a hands-on or visual learner? Each chapter starts with one (or more) hands-on exercise to get you familiar with the scientific concepts in the chapter. The second half is the "Fundamentals" section, which goes in-deep into the heart of the matter with examples, analogies, colored illustrations, diagrams, all the while linking back to the hands-on exercises, giving you that solid, practical knowledge you can use. See our blog article with in-depth details about the  Zero to Genetic Engineering Hero guide book and learning journey. 

With NGSS and Alberta curriculum alignment, our lesson guides developed by Mindfuel.ca (Alberta Science Foundation) make it easy for educators to implement hands-on student activities in the classroom based on the genetic engineering concepts presented in Amino Labs kits and their Zero to Genetic Engineering Hero Guide book.

Each lesson guide includes a variety of student activities such as hands-on experiments, virtual simulators, individual and group assignments, powerpoint presentations, worksheets and assessments with accompanying teacher keys. These covers: Discovering DNA, Genetic Engineering & Lab safety, (Free download - teach & practice safe science ☺︎) Growing and understanding E. Coli cells, and Genetic Engineering E. Coli cells with DNA Plasmids.  

How do the kits work?

Amino Lab's Experiment Kits make it easy to learn the fundamentals of growing, engineering and manipulating cells. For example, with the Engineer-it kit and many of the advanced kits, you can easily add a DNA program into living cells, grow and take care of those cells so that they can produce something for you—light, pigments, enzymes, materials, and more! 

All the components in the kits are pre-measured and labeled for ease-of-use. Illustrated manuals are available on our instruction manual page, and we also have follow-along videos on our Youtube channel. The kits can be combined to get an end-to-end bioengineering experience. You will find detailed information for each kit in the different sections of the manuals and the theory behind the experiments in the Zero to Genetic Engineering Hero book.

For example, here is an overview of how some of the kits work together: using an Engineer-it kit and Extract-it (Plate) Kit you can:

  • take the provided colour-generating DNA program (inspired by corals) and insert it into bacteria,
  • amplify the bacteria to obtain a lot of pigment-producing bacteria, 
  • extract and purify the pigments grown in the bacteria, getting rid of the bacteria
  • paint or dye things with your bio-pigment!
What is DIYBio (Do-It-Yourself Biology)?

Do-it-yourself biology (DIY biologyDIY bio) is a growing biotechnological social movement in which individuals, communities, and small organizations study biology and life science using the similar methods as traditional research institutions. Traditionally, DIY biology is undertaken by individuals with extensive research training from academia or corporations, who then mentor and oversee other DIY biologists with little or no formal training. This may be done as a hobby, as a not-for-profit endeavor for community learning and open-science innovation, or for profit, to start a business.  

With Amino Labs kits and tutorials, this need for a skilled mentor, which can be hard to find, has been removed; let the kits be your mentors!

Popular media articles about genetic engineering at home

STEM Programs That Will Attract Kids to Science and Engineering Careers: http://www.universityherald.com/articles/47016/20161101/stem-programs-science-and-engineering-careers.htm

This Playful Lab-in-a-Box Will Teach You How to Reprogram Life: https://singularityhub.com/2016/12/28/this-playful-lab-in-a-box-will-teach-you-how-to-reprogram-life/

These Programs Want Kids To Be Bioengineers And Scientists: https://www.forbes.com/sites/jenniferhicks/2016/10/29/these-programs-want-kids-to-be-bioengineers-and-scientists/#2681bd3c6fd4

BIOFABRICATE: Biotech and Synbio for the Production of New Sustainable Materials: http://blogs.plos.org/synbio/2016/11/24/biofabricate-biotech-and-synbio-for-the-production-of-new-sustainable-materials/

Now You Can Genetically Engineer Living Cells with a Home Kit. Should You?: https://www.technologyreview.com/s/543491/now-you-can-genetically-engineer-living-cells-with-a-home-kit-should-you/

Amino-a countertop bioreactor making bioengineering accessible to everyone!: https://biotechin.asia/2015/11/06/amino-a-countertop-sized-bioreactor-making-bioengineering-accessible-to-everyone/

The Synthetic Biology Era Is Here—How We Can Make the Most of It: https://singularityhub.com/2016/08/19/the-synthetic-biology-era-is-here-how-we-can-make-the-most-of-it/

Tools for Would-Be Biohackers: Here Come 3 Mini-Labs: http://spectrum.ieee.org/the-human-os/biomedical/devices/tools-for-would-be-biohackers-here-come-3-mini-labs

Biohacking and the Do-It-Yourself Biology revolution: https://biotechin.asia/2016/02/23/biohacking-and-the-do-it-yourself-biology-revolution/

Homebrew Biology Kit Brings Synthetic Biology To The Masses: https://www.newscientist.com/article/2076666-homebrew-biology-kit-brings-synthetic-biology-to-the-masses/

Germany Is Threatening Biohackers With Prison: http://gizmodo.com/germany-is-threatening-biohackers-with-prison-1792143993

Synbio and biosecurity: https://phys.org/news/2017-02-synbio-biosecurity.html

Experiments Kits

General experiment kits FAQs

Where can I find the instructions for my kit?
How do I dispose of my experiment materials responsibly?

Each experiment kit that has living organisms or tubes of DNA and antibiotics includes an inactivation bag and instructions on how to dispose of your materials safely and responsibly. You will need chlorinated bleach to complete this step, whcih we do not provide due to shipping regulations. If you are at a school or lab that uses a different type of inactivation cleaner than bleach like 70% ethanol, or a biocidal cleaner (it will say so on the bottle), those are fine too.

If you have a question about your school or lab's cleaner, or any safety procedures, don't hesitate to reach out.

You can get extra inactivation bags for your independant experiments here: inactivation bags

What safety supplies do I need?

Depending on the experiment you are completing, you may need gloves, an apron/lab coat*, and a chlorinated bleach cleaner or isopropyl alcohol**. Protective eyewear is not necessary but can be worn if you want to, of course.

You'll find a list of the necessary supplies under the "What else is needed" tab in the product description on each product page, and in each kit's instruction manual.

* The apron or lab coat is used to protect clothing when using the chlorinated bleach cleaner, and as part of establishing good safe science habits. It is not strictly necessary.

** If your school or lab already uses a different type of inactivation cleaner than bleach like 70% ethanol, or a biocidal cleaner (it will say so on the bottle), those are good too.

I don't have a lab. What equipment do I need for these experiment kits?

To complete Amino Labs experiements, you do not need to have a special lab in your school or your home. You can set up on any surface that can be wiped clean.

While each experiment will have its own requirements in terms of equipment, they are designed to build on each other. The beginner experiments will need no equipment, while the most advanced may need a few pieces of equipment.

For our beginner kit, the DNA Extraction Kit, you do not need any equipment.

For our most popular kit, the Canvas Kit, you only need to have access to :

  • a microwave (it can be a microwave used for food since only sterile water bottles will go in)
  • optional: an incubator that reaches 37 °C (regular Canvas Kit) or 30°C (Yeast Canvas Kit). If you do not have an incubator, the color in the agar art paintings will not be as bright and will take longer to grow, but will still work! You can even try your hand at building your own incubator

For the Engineer-it Kit experiment, we recommend having the DNA Playground for best results and less mess. But you can also use D.I.Y. equipment for these experiments. Note that your results will depend on how accurate your equipment is! If you do not have the DNA Playground you will need

For the Plate Extract-it Kit and The Blue-it Kit, you would need the equipment above, plus a microcentrifuge.

So, to complete the full Genetic Engineering Hero Journey, you would only need access to a microwave, a DNA playground (or the replacement thermometer, ice, hot water, and an incubator/DIY incubator), and a microcentrifuge.

You can have a look on each experiment kit's product page under the What else is needed tab or in the intruction manuals to find out what each kit requires.

What is the difference between an "Individual" and a "Group" kit?

Most kits comes in Individual size and Group/Classroom size. These kits contains the same ingredients in different quantities to allow you to complete one (individual size) or more (group size) of the same experiment.

The Individual Kit: an individual kit will include everything required to complete the experiment once. This can be done alone, in a small group or as a demonstration in front of a group.

The Group Kit: a group kit will include everything needed to complete the experiment 4 or 8 times, depending on the kit. That means a classroom or group can all perform the same experiment with a group kit. If you are working alone, you can also get a group kit if you know you'd like to repeat the same experiment a few times, like for a science fair project, or for honing your skills. Each Kit's product page will state how many experiments can be done with a Group kit, between 4 (advanced kits) and 8 (beginner kits)

Can I do these kits at home?

In North America, yes! Our kits are Biosafety Level 1 / Risk Group 1 which means they do not require special containment in North America. 

In Europe, however, there are different containment laws by country. Please check with your local authorities or find a local certified DIYBio space. You can start by looking here https://diybio.org/local/ 

Europe is well connected so you may want to take a weekend biohacking vacation to DIYbio spaces such as La Paillasse in Paris, MadLab in Manchester, London Biohackers in London, Waag Society in Amsterdam or many others.

If you purchase a Zero to Genetic Engineering Hero starter pacl, you can take it to one of these spaces and do the experiment! They'll probably love to join you!

What is the bacteria in the kits?

We use lab-strain, non-pathogenic E.Coli bacteria. Many people hear of E. coli and think pathogen! In fact, most if not all humans have E. coli naturally occurring in our large intestines. E. coli is critical in helping to maintain gut health. About 100 years ago some E. coli was collected and was analysed in a lab. This "strain" was shared by more and more labs. Through that process, scientists, slightly modified the bacteria to make them more "lab friendly". One hundred years later, tens of thousands of labs have used "lab strains" of E. coli for research, biomanufacturing, and more! 

Lab strains of E. coli have modifications to them that make it virtually impossible for them to live in the environment, Amino Labs offers "K12 E. coli".

You can learn more here. If you want to discover to history of the lab E. coli and take a tour of its inner working, Chapter 3 of Zero to Genetic Engineering Hero is for you!

What is the timeline for completing an experiment?

Each experiment will have its own timeline, between one day and 12 days, and most will need to be completed on consecutive days since bacteria grow on their own schedule! Have a look at each kit's product page for a quick glance at the timeline, and in the instruction manuals for a detail explanation.

Some experiments, like the Canvas Kit and the Engineer-it Kit can be completed with some timing "hacks" to fit within a specific schedule you might be working with, like in school, club or in summer camp. If this is the case, these will be outlined in the instruction manuals, and you can always check with us before by contacting us.

For example, there are 2 timelines for running an Engineer-it Kit experiment: the 4-day recommended timeline, and the 3-day timeline.  Each timeline requires consecutive experiment days, but, in the instruction manual, you'll also find a few tips and trick to modify the timeline to wait a week between each "day" of experiment should you need it. Have a look! 

How do I troubleshoot experiment or kit issues?

Try the Troubleshooting Guide:

Visit https://amino.bio/pages/success-guarantee where you can launch and use a simple application that will help you to identify what went wrong and get some insights into your experiment.

How long are kits good for once I receive them?

All kits that expire will have a "Best by" sticker on the label. The sticker will say "Best by the end of: Month/Year". Use your kits before the end of the Month on the sticker for best results. If you can't use the kits by their best-by date, we have replacement bacteria available so your kits can still be used: the only items that will expire within those months are the bacteria - the rest of the items, like the agar and selection tablets, are good for at least a year. 

We ship our kits so that they have at least 3 months' lifespan from when you receive them. Once you receive your order, place the kit(s) in the refrigerator according to the storage information card in your box. 

Kits that don't have a "Best by" sticker, like the Bioplastic Kits or the DNA Extraction Kit, do not expire if stored properly. You can use them at any time. 

Experiment-specific FAQs

What does the Engineer-it kit allow you to do?

Cells are tiny living units that function like mini-factories. Bacteria are single-celled organisms. Individual bacteria can only be seen with a microscope, but they reproduce so rapidly that they often form colonies that we can see. Bacteria reproduce when one cell splits into two cells through a process called binary fission. Fission occurs rapidly, in as little as 20 minutes. Under perfect conditions a single bacterium could grow into over one billion bacteria in only 10 hours!

Each bacteria, or cell, is told how to use its factory-like capabilities by its DNA. DNA is like a computer program; it is the set of instructions that tells the cell(s) how to function. 

In this kit, you get a DNA plasmid (we will sometime refer to it as a DNA Program since it is an easier language to grasp). DNA plasmids are also a set of instructions for the cells, but much smaller—a plasmid only has a few functions, as opposed to the complete genome of the bacteria. Bacteria share vital information with each other by passing along plasmids. By inserting a new plasmid in our bacteria, we can get them to produce things for us. In the case of our Magenta Engineer-it Kit, the plasmid encodes for the creation of Magenta-coloured pigments.

Since DNA is a very hydrophilic molecule, it won’t normally pass through the bacteria's cell membrane. In order to make bacteria take in the plasmid, it must first be made “competent”. This means creating small holes in the bacterial cells by suspending them in a solution with a high concentration of calcium (the transformation buffer). DNA can then be forced into the cells by incubating the cells and the DNA together on ice, placing them briefly at 42°C (heat shock), and then putting them back on ice. This causes the bacteria to take in the DNA and is called “Transformation”. 

You will need to grow your bacteria on the nutrient agar petri dish (plates) before you insert the DNA plasmid. Freshly grown bacteria take up DNA much better than older ones since they are still in a growth phase. Nutrient agar is a jello-like staple food source for the bacteria which you will pour into the provided plates (petri dish) in the first step. Two types of agar will be made: non-selective and selective. The non-selective agar allows any bacteria to grow, while the selective agar has an antibiotic mixed in which allows only antibiotic-resistant bacteria to grow. The DNA plasmid you insert will make your bacteria antibiotic-resistant so that only those cells will grow. 

Specifically, your Engineer-it Kit will allow you to complete the following hands-on exercises to successfully achieve a bacterial transformation : 

1. Make selective and non-selective plates for growing bacteria.
2. Grow/streak K12 E. coli (to be used for DNA transformation)
3. Make K12 E. coli cells chemically competent (able to take up DNA plasmids)
4. Transform the competent cells with DNA program(s) (i.e. for generating colour pigments)
5. Recover and grow the transformed cells on plates

Try Virtual Bioengineer simulation to get a comprehensive understanding of how an Engineer-it Kit and DNA Playground work enable you to engineer cells. Watch some tutorials on Youtube.

How do I complete an Engineer-it Kit without an Amino Labs' DNA Playground?

If you don't have a DNA Playground, you will need to get:

  • Ice and Ice Bucket
  • Warm water bath (can be done using a bowl and manually setting water temperature)
  • Thermometer
  • Incubator to incubate cell and plates -- this needs hold 37C degrees. You can even make your own

See the full instructions in our instruction manuals and in this online course

In the Extract-it-Plate kit, can I streak one plate with one color of Engineered bacteria and one plate with another color?

Absolutely! You can try to mix your colors during the pigment extractions. Of course, this might not be how scientists would do it in the lab (they would more than likely extract each color separately) but we've done it in our lab, and it can create some fun results, especially if you mix a non-fluorescent color with a fluorescent one! 

Have fun!

I want to do the Canvas Kit with my students, but it will be the week before spring break. Can I let the plates (Day 2) grow at room temperature for a full week? Or will they overgrow? Would they still grow & express in left in the refrigerator?

If you let the plates grow at room temperature for a week,. they will not overgrow! But growing at room temperature (and not 37C) will give you pastel versions of the color. In other words, the colors will not get as bright as if you incubate them at 37C, but they will still be beautiful. Have a look at the image below to see the difference in colors. Don't forget to place the petri dishes in a closed, resealable bag while they incubate at room temperature so that they don't dry out!  

In the refrigerator, they would unfortunately not grow - but you could use the refrigerator in another way: If you have a 37C incubator, you can incubate the painting palettes for 24 to 36 hours before spring break, and then pop them in a resealable bag or tupperware-style plastic container, place them in a refrigerator and that will keep them fresh for you to use with the students the following week (or many weeks later!) 

If you don't have an incubator, you can have a look at this simple tutorial on how to make a quick one with items from around the house or classroom. https://www.youtube.com/watch?v=LEsv0Qvbczs 

How many petri dish can I preserve with one Keep-it kit?

With the Individual size Keep-it kit, you can preserve up to 3x 6 cm petri dish. 6cm petri dish are the ones included in all Amino Labs experiment kit.  If you want to preserve the larger 10 cm petri dishes, we recommend 1 Keep-it kit per 10 cm petri dish. 

With the Group size Keep-it kit, you can preserve up to 8x more petri dishes than with the Individual kit. That means in one group kit you will receive 8 individual kits! So, up to 24x  6 cm petri dishes can be preserved. If you want to preserve the larger 10 cm petri dishes, you will be able to preserve 8 of them.  

Note that the kit comes with petri dish stands made for 6 cm petri dishes. If you are preserving 10 cm petri dishes, email us with your order number so we can swap out the stands for one to fit your larger petri dish!

I only got one engineered colony on my S(e) plate in the Engineer-it Kit. How can I get more next time?

In genetic engineering, practice makes perfect! Having even one engineered colony on your experiment plate is a success! Often times, one colony is all a scientist really needs to continue their experiment, so congratulations!

It is actually very likely that you will get more colonies next time, as your skills and understanding of each step of the experiment gets better. Another way to maximize your chances of getting a lot of engineered colonies is to leave your engineered cells to recover for as long as possible, up to the 24 hour mark. 

Finally, to get more colonies next time, you can also practice with these resources to really hone your skills and knowledge:

Equipment

What is the DNA Playground?

The DNA Playground™ is a simple and engaging science station that enables you to genetically engineer bacteria, and to incubate and grow your bacteria. Small, contained and safe, this station is a new bioengineer's must! Watch tutorial videos on Youtube to see it in action.

It can be used at home or by educators in schools as an easy alternative to traditional and messy lab equipment. With a class setup time of under 10 minutes, the station allows you to get started with your genetic and biological engineering right away!

Each DNA Playground Large can do up to 4 simultaneous transformations and replaces the need for heated water baths, ice buckets and ice, incubator, timer and thermometers. It complements the Next Generation Science Standards (NGSS) and is compatible with most bacterial transformation kits available on the market.  

Learn more here

If you already have your own lab, you can use an incubator, heat bath and crushed ice to complete the experiments without the DNA Playground.

Where can I find the instructions for my Amino Labs equipment?
I do not have a lab at school/home. Can the experiments still be completed?

Absolutely. To complete Amino Labs experiements, you do not need to have a special lab in your school or your home.

For our most popular kit, the Canvas Kit, you only need to have access to :

  • a microwave (it can be a microwave used for food since only sterile water bottles will go in)
  • surfaces that can be wiped clean to work on
  • optional: an incubator that reaches 37 °C (regular Canvas Kit) or 30°C (Yeast Canvas Kit). If you do not have an incubator, the color in the agar art paintings will not be as bright and will take longer to grow, but will still work! You can even try your hand at building your own incubator

For the Engineer-it Kit experiment, we recommend having the DNA Playground for best results and less mess. But you can also use D.I.Y. equipment for these experiments. Note that your results will depend on how accurate your equipment is! If you do not have the DNA Playground you will need

You can have a look on each experiment kit's product page under the What else is needed tab or in the intruction manuals to find out what each kit requires.

What lab equipment is needed for me to use Amino Labs as a science elective for 12th grade seniors? In other words, do you have a required lab equipment list?

The equipment needs depends on which of our kits you will be using.

For example...

For the Canvas Kit to make living paintings (agar art) you need access to a microwave and a 37C incubator. Students can build one in class, you can use existing ones you may have, or you can forgo the incubator and grow the bacteria at room temperature, in which case the bacteria will not be as brightly color, but will still grow. 

For the Engineer-it kit (genetically engineer bacteria to make colors), the Smell-it kit (genetically engineer bacteria to make enzymatic reactions that smell), the Induce-it Kit (genetically engineer bacteria and control the genetic circuit with chemicals), the Heat-it kit (genetically engineer bacteria and control the genetic circuit with chemicals), you need access to a microwave, the 37C incubator, ice, a water bath or hot water and temperature that reaches 37C and 42C. We also have our own all-in one minilab, the DNA Playground, which covers all these. 

For the Extract-it kit (to extract the pigment colors of the engineered bacteria from the cells)  and the Blue-it kit (genetically engineer bacteria to make enzymatic reactions that does color-change) you need the same as above plus a micro-centrifuge that spins at at least 13,000 x g.   

You can have a look on each experiment kit's product page under the What else is needed tab or in the intruction manuals to find out what each kit requires. Or reach out to us and we'll be happy to talk you through it.

Virtual Simulators

The virtual simulator is stuck - I can't drag or click anything!

A few problems can arise when using the simulators: 

  • You are trying to use the simulators on mobile. Sorry! Our simulators are not yet optimized for mobile device use - you need to be on a computer browser to use them. We are working on adding mobile-capabilities. 

  • You have a pop-up blocker or similar enabled. For certain drag and drop actions, a pop-up blocker seems to cause issues. Try to disable it for the simulator. 

  • The simulator is still loading in the background. The timing of the actions can be off if you have a slow internet connections. In general by the time you read the text on the screen, the simulator is ready for you to perform the action. If nothing happens and you know you are performing the right action (more on that below), then wait a up to a minute and re-try. Hopefully the simualtor is done loading by then!

  • You are not doing the action required by the simulator... for example, you may need to be "mixing" your agar into your water bottle by clicking and draggin the bottle up and down on the screen for a few seconds. Have a look at our simulator videos below. They are like a cheat sheet for the actions! 

Teaching Resources

Where can I find the instructions for my kit or equipment?
I want to access the Google Classroom for Educators, but I am getting an error message

Unfortunately, if you are trying to access our Google Classroom using your school email, and get an error message, it likely means that your email is part of your school/institution Google education account, which prevents access to outside classrooms. This is a setting that can't be changed due to privacy and security settings at your institution level.

If you have a personal email we recommend you log in that way. In the meantime, we are working to find a solution for the 2023-2024 school year, and onwards. We appreciate your patience, and are happy to hear from you if you need help.

I want to teach my students about ...... , do you have a resource for that?

Have a look at our Google Classroom for Educators and our blog to see if the resource already exists. If you are looking for plasmid maps, or a resource we don't have, reach out.

If you want to contribute a resource you created, amazing! Let us know.

I completed the exercises in the book and the "end of chapter" questions. How can I verify my answers?

Congratulations! Reach out to us for the answer keys!

Ordering

What supplies do I need?

If you are interested in completing an experiment kit, and are wondering what equipment and safety supplies you will need, have a look at the product description on the kit's product page (under the photos). You'll see a tab called "What's included" and "What else is needed". These tabs should contain all the information you need, including any safety supplies you'll want to have on hand, like gloves and cleaner.

We know that getting started with something new can be a bit daunting, and we're here to help. So if you still have any questions at all, or want to see if your own existing equipment is up to the task, don't hesitate to use the live chat bubble in the bottom right corner or contact us page to reach out.

Can I cancel my order?

We completely get it, we change our minds too! Please contact us right away if you wish to cancel or change your order. Once an order has shipped, unfortunately, we are unable to alter or cancel it at this time.

Should the item(s) not work out, make sure to reach out.

Do you accept Purchase Orders (P.O.s)?

Absolutely! Please send your P.O.s to orders (at) amino.bio.

You can also use this email to request quote(s), vendor information or to have us fill out your vendor forms.

Educational Purchases

Can you help me figure out what I need?

Absolutely! Contact us and one of our experts will be happy to discuss how we can help you reach your goals, from free resources to custom kits and lessons.

We'll be happy to speak over email, video chat and phone call. Let us know your preference when you reach out.

Do you accept Purchase Orders (P.O.s)?

Absolutely! Please send your P.O.s to orders (at) amino.bio.

You can also use this email to request quote(s), vendor information or to have us fill out your vendor forms.

Can you become an authorized vendor for my school/disctrict/city?

Of course. Please send us the vendor forms to fill out, or a link to the vendor registration process, and we'll be happy to complete the process.

Please use our orders (at) amino.bio email.

We do not have a lab. Can I still have my student complete the experiment(s)?

Absolutely. To complete Amino Labs experiements, you do not need to have a special lab in your school.

For our most popular classroom kit, the Canvas Kit, you only need to have access to :

  • a microwave (it can be a microwave used for food since only sterile water bottles will go in)
  • surfaces that can be wiped clean for the students to work on
  • optional: an incubator that reaches 37 °C (regular Canvas Kit) or 30°C (Yeast Canvas Kit). If you do not have an incubator, the color in the agar art paintings will not be as bright and will take longer to grow, but will still work! You can even try your hand at building your own incubator

For the Engineer-it Kit experiment, we recommend having the DNA Playground for best results and less mess. But you can also use D.I.Y. equipment for these experiments. Note that your results will depend on how accurate your equipment is! If you do not have the DNA Playground you will need

  • Thermometer that can measure 37 °C and 42 °C
  • Cup of hot water
  • Cup of ice (crushed ice is best!)
  • incubator that can reach 37 °C (like this one, or one you/your students make.

Have a look on each experiment kit's product page under the What else is needed tab or in the intruction manuals to find out what each kit requires.

Shipping & Exchanges

We use all major carriers, and local courier partners. You’ll be asked to select a delivery method during checkout.

What is the shipping policy?

Once your order is placed, you will receive a confirmation email letting you know that our fulfillment team has received your order. Once your order is fulfilled, you will receive an email notification with your tracking information. Shipping fees are non-refundable in the case of returns.

Do you ship internationally?

Yes, we ship worldwide from our manufacturing headquarters in Canada and the US. Shipping costs will apply, and will be added at checkout.

When shipping to international locations, we do not pre-collect taxes on your order and you may need to pay brokerage / custom fees to fulfill your countries taxation laws.  We identify our packages as "Educational Science Kits" in custom forms. 

We can provide you with MSDSs if your customs agent required them. Please contact us for this or other assistance!

Please note that if you are outside of North America, your country may have restrictions regarding genetic engineering activities in the home or school outside of a regulated lab. In Europe for example, each country has specific requirements. To learn more about your country's requirement, please check with your local authorities or find a local DIYBio space. You can start by looking here https://diybio.org/local/ 

For countries where we know the regulations prohibit you from doing experiments outside of a lab, we have disabled shipping at checkout. If you are in one of the countries that does not show up upon checkout, but have access to a lab space to work in, please contact us. We'll be happy to help you get the products you want for your lab.

My country is not recognize when I try to check out.

Your country may have special regulations and requires us to verify your acknowledgment of them before shipping. Some countries may even require us to send your kit directly to an approved lab space, like a school, university or industry lab, or community space.

Or, we simply haven't shipped there yet, and this is an oversight which is easily fixed.

Please contact us

How long does it take for orders to be fulfilled?

Typically products are shipped 1-10 working days after you place your order. For product-specific fulfillment times, have a look on the product page under "Storage & Shipping."

If you require your order for a specific date or event, please contact us before or as soon as possible with your order # so that we can try to make it happen for you! ☺︎ 

Safety

Is it safe?

Amino Labs kits are optimized for use with a friendly type of bacteria and yeasts that has been used by scientists for over 100 years.

Organisms, which do not cause diseases are called non-pathogenic, and all the the bacteria and yeast we used in all our kits are non-pathogenic. They are part of what is called the BioSafety Level 1 / Risk Group 1 Classification. This is the lowest classification group, and is meant for the organisms that are not known to cause disease in healthy adults. Read more about the why it is considered safe in our blog.

If you or anyone else in your home/class is immunocompromised, you may need aditional precautions when completing the experiments. Always read through and follow the safety guidelines in the manual. You can have a look at our Practicing Safe Science guidelines to see general safety practices, gather safety resources and complete safe science quizzes and checklists before your get going!

(Please check your local regulations if you are outside of North America as these might be different. Contact us at info@amino.bio for any questions)

There are always challenges around the subject of biosafety and biosecurity. Amino Labs has aligned itself with the National Security Counsel, and The National Strategy for Countering Biological Threats, who have asserted that "The beneficial nature of life science research is reflected in the widespread manner in which it occurs. From cutting-edge academic institutes, to industrial research centers, to private laboratories in basements and garages, progress is increasingly driven by innovation and open access to the insights and materials needed to advance individual initiatives." Moreover their strategy is targeted to reduce biology threads by "improving global access to the life science to combat infectious disease regardless of its cause".  Our lead Scientist, Dr. Justin Pahara, is an Emerging Leaders in Biosecurity Initiative fellow and will make sure Amino Labs is pioneering accessible and safe hands-on biology.  

We believe that increasing meaningful engagement with and understanding of biology can only be done in a learn-by-doing manner, and this means making biotechnology available. This will naturally create concern and we are here to help with any further questions, should you have any.

Can I do these experiment kits at home?

Great question! In North America, yes, absolutely. Our kits are Biosafety Level 1 / Risk Group 1 which means they do not require special containment (space) in North America. 

In Europe, however, there are different containment laws by country since our kits enable you to complete genetic engineering experiments. Please check with your local authorities or find a local DIYBio space. You can start by looking here https://diybio.org/local/

Practicing Safe Science Guidelines

Have a look at the general Safe Science guidelines below. Relevant safe science guidelines will also be found in each instruction manuals. 

Bioengineering, genetic engineering and life sciences are safe activities when you follow simple guidelines. Read on to ensure you adopt safe practices. 

Firstly, the kits contains only non-pathogenic ingredients. These are rated Risk Group 1 (RG1) (Biosafety Level 1), the most benign level (and therefore the safest). With these ingredients, no special containment or training is required in North America*, but you must follow the following rules for your safety and the safety and success of your experiment(s)! We recommend the system for ages 12+, under adult supervision, and 16+ unsupervised.

We recommend that the discard container be emptied by an adult and that the cleaning instructions be strictly followed for safety and experiment success. Make sure to store the ingredients in accordance with the instructions found in this booklet (refrigerated, in the freezer, or room temperature). 

  • Eyewear is not provided but should be worn. 
  • Do not eat or drink near your experiments. Keep your experiment at least 10 feet from food, drinks, etc. Under no circumstances should you consume any of the ingredients. 
  • Immunocompromised persons: While the ingredients in these kits are considered non-pathogenic, some persons, such as immunocompromised persons, can be affected by large numbers of bacteria and should wear extra protection to ensure no contact with the ingredients. 
  • Wash your hands before and after manipulating your experiment, the ingredients, or the hardware. 
  • Wear gloves, even when cleaning your station or handling the consumables (petri plates, loops, etc). This will protect you from your experiment, and your experiment from you. Any latex, nitrile, or general purpose gloves you can find at the pharmacy will do. Also, after you put your gloves on, be aware of what you touch. Try not to touch your face, scratch itches with your gloved fingers! 
  • Make sure all hardware is place on a solid surface, away from young children and pets. Keep it level at all times.  
  •  Clean up your station, spills and work surface before and after use. Use a 10% solution of chlorinated bleach generously sprayed onto a paper towel and rub onto any contaminated surfaces. (Careful! This can discolor your clothes). A chlorinated spay cleaner will also work. 
  • Find a container to discard used consumables such as the blue and yellow loops, and tubes. An old large yogurt container, large plastic cup or the like will do. You will then fill this with bleach once your experiment is over, as described in the "After your experiment" section of the instruction manuals.

Note that instruction manuals will include the most up to date and experiment-specific safety information. Always read the safety guidelines in your manual before starting an experiment. 

If you would like to complete a quiz to make sure you've understood the guidelines, you can complete one here before completing any experiments. www.amino.bio/biosafety-quiz

You can also download these fun biosafety and biosecurity posters for your lab or classroom. These posters are shared courtesy of the Public Health Agency of Canada to supports new scientists! www.amnio.bio/whatisbiosafetywww.amino.bio/biosafetyinaction

Finally, you can use our safety checklist before, during and after each experiment. This checklist was created as a supplement to chapter 2 of the Zero to Genetic Engineering Hero Beginner's guide book, but you can use it without the book. Download the safety checklist www.amino.bio/checklist

If you would like to do a short online lab safety course for your edification, we recommend this Government of Canada course: https://training-formation.phac-aspc.gc.ca/

For the full list of safe science resources we have, head over to our Safe Science blogs

Is the antibiotic we add to the petri dish in some experiments going to create antibiotic resistance?

Good question! Of course, at Amino Labs, we take safety seriously and we've addressed the use of antibiotics in the experiments and antibiotics resistance in this blog article: https://amino.bio/blogs/news/engineer-it-kit-safety

You can also find more information in the Zero to Genetic Engineering hero book chapter 3. 

Do you sell safety supplies?

We do! We have mini safety sets for home and classroom use that include nitrile gloves and reusable, wipeable aprons. You can add these to your cart seperately, or include one in the Zero to Genetic Engineering Hero pack options when you add to cart.

Each experiment kit that has living organisms or tubes of DNA and antibiotics includes an inactivation bag and instructions on how to dispose of your materials safely and responsibly. You will need chlorinated bleach to complete this step, whcih we do not provide due to shipping regulations. If you are at a school or lab that uses a different type of inactivation cleaner than bleach like 70% ethanol, or a biocidal cleaner (it will say so on the bottle), those are fine too.

If you have a question about your school or lab's cleaner, or any safety procedures, don't hesitate to reach out.

How do I dispose of my experiment materials responsibly?

Each experiment kit that has living organisms or tubes of DNA and antibiotics includes an inactivation bag and instructions on how to dispose of your materials safely and responsibly. You will need chlorinated bleach to complete this step, whcih we do not provide due to shipping regulations. If you are at a school or lab that uses a different type of inactivation cleaner than bleach like 70% ethanol, or a biocidal cleaner (it will say so on the bottle), those are fine too.

If you have a question about your school or lab's cleaner, or any safety procedures, don't hesitate to reach out.

You can get extra inactivation bags for your independant experiments here: inactivation bags